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Publications of Pfanner Lab


  • Krüger V, Becker T, Becker L, Montilla-Martinez M, Ellenrieder L, Vögtle FN, Meyer HE, Ryan MT, Wiedemann N, Warscheid B, Pfanner N, Wagner R, Meisinger C: Identification of new channels by systematic analysis of the mitochondrial outer membrane. J Cell Biol, 2017; 216: epub September 15, 2017.
  • Wiedemann N, Pfanner N: Mitochondrial machineries for protein import and assembly. Annu Rev Biochem, 2017; 86: 685-714; epub March 15, 2017.
  • Hessenberger M, Zerbes RM, Rampelt H, Kunz S, Xavier AH, Purfürst B, Lilie H, Pfanner N, van der Laan M, Daumke O: Regulated membrane remodeling by Mic60 controls formation of mitochondrial crista junctions. Nat Commun, 2017; 8: 15258.
  • Rampelt H, Bohnert M, Zerbes RM, Horvath SE, Warscheid B, Pfanner N, van der Laan, M: Mic10, a core subunit of the mitochondrial contact site and cristae organizing system, interacts with the dimeric F1Fo-ATP synthase. J Mol Biol, 2017; 429: 1162-1170.
  • Rampelt H, Zerbes RM, van der Laan M, Pfanner N: Role of the mitochondrial contact site and cristae organizing system in membrane architecture and dynamics. Biochim Biophys Acta, 2017; 1864: 737-746.
  • Morgenstern M, Stiller SB, Lübbert P, Peikert CD, Dannenmaier S, Drepper F, Weill U, Höß P, Feuerstein R, Gebert M, Bohnert M, van der Laan M, Schuldiner M, Schütze C, Oeljeklaus S, Pfanner N, Wiedemann N, Warscheid B: Definition of a high-confidence mitochondrial proteome at quantitative scale. Cell Rep, 2017; 19: 2836-2852


  • Ellenrieder L, Opalinski L, Becker L, Krüger V, Mirus O, Straub SP, Ebell K, Flinner N, Stiller SB, Guiard B, Meisinger C, Wiedemann N, Schleiff E, Wagner R, Pfanner N, Becker T: Separating mitochondrial protein assembly and endoplasmic reticulum tethering by selective coupling of Mdm10. Nat Commun, 2016; 7: 13021.
  • Rampelt H, Pfanner N: Coordination of two genomes by mitochondrial translational plasticity. Cell, 2016; 167: 308-310.
  • Chatterjee A, Syefferth J, Lucci J, Gilsbach R, Preissl S, Böttinger L, Martensson CU, Panhale A, Stehle T, Kretz O, Sahyoun AH, Avilov S, Eimer S, Hein L, Pfanner N, Becker T, Akhtar A: MOF acetyl transferase regulates transcription and respiration in mitochondria. Cell, 2016; 167: 722-738.
  • Stiller, S.B., Höpker, J., Oeljeklaus, S., Schütze, C., Schrempp, S.G., Vent-Schmidt, J., Horvath, S.E., Frazier, A.E., Gebert, N., van der Laan, M., Bohnert, M., Warscheid, B., Pfanner, N., and Wiedemann, N: Mitochondrial OXA translocase plays a major role in biogenesis of inner-membrane proteins. Cell Metab, 2016; 23: 901-908.
  • Zerbes, R.M., Höß, P., Pfanner, N., van der Laan, M., and Bohnert, M: Distinct roles of Mic12 and Mic27 in the mitochondrial contact site and cristae organizing system. J Mol Biol, 2016; 428: 1485-1492.
  • Straub, S.P., Stiller, S.B., Wiedemann, N., and Pfanner, N: Dynamic organization of the mitochondrial protein import machinery. Biol. Chem, 2016; 397: 1097-1114.
  • van der Laan, M., Horvath, S.E., and Pfanner, N: Mitochondrial contact site and cristae organizing system. Curr Opin Cell Biol, 2016; 41: 33-42.



  • Bohnert M, Zerbes RM, Davies KM, Mühleip AW, Rampelt H, Horvath SE, Boenke T, Kram A, Perschil I, Veenhuis M, Kühlbrandt W, van der Klei IJ, Pfanner N, van der Laan M: Central role of Mic10 in the mitochondrial contact site and cristae organizing system. Cell Metab, 2015; 21: 747-755.
  • Horvath SE, Rampelt H, Oeljeklaus S, Warscheid B, van der Laan M, Pfanner N: Role of membrane contact sites in protein import into mitochondria. Protein Sci, 2015; 24: 277-297.
  • Wenz LS, Ellenrieder L, Qiu J, Bohnert M, Zufall N, van der Laan M, Pfanner N, Wiedemann N, Becker T: Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting β-barrel biogenesis. J Cell Biol, 2015; 210, 1047-1054.
  • Bohnert M, Pfanner N, van der Laan M: Mitochondrial machineries for insertion of membrane proteins. Curr Opin Struct Biol, 2015; 33, 92-102.
  • Shiota T, Imai K, Qiu J, Hewitt VL, Tan K, Shen HH, Sakiyama N, Fukasawa Y, Hayat S, Kamiya M, Elofsson A, Tomii K, Horton P, Wiedemann N, Pfanner N, Lithgow T, Endo T: Molecular architecture of the active mitochondrial protein gate. Science, 2015; 349, 1544-1548.



  • Harbauer AB, Opalinska M, Gerbeth C, Herman JS, Rao S, Schönfisch B, Guiard B, Schmidt O, Pfanner N, Meisinger C: Cell cycle-dependent regulation of mitochondrial preprotein translocase. Science, 2014; 346: 1109-1113.
  • Ieva R, Schrempp SG, Opalinski L, Wollweber F, Höß P, Heißwolf AK, Gebert M, Zhang Y, Guiard B, Rospert S, Becker T, Chacinska A, Pfanner N, van der Laan M: Mgr2 functions as lateral gatekeeper for preprotein sorting in the mitochondrial inner membrane. Mol. Cell, 2014; 56: 641-652.
  • Opalinski L, Becker T, Pfanner N: Clearing tail-anchored proteins from mitochondria. Proc Natl Acad Sci USA, 2014; 111: 7888-7889.
  • Harbauer AB, Zahedi RP, Sickmann A, Pfanner N, Meisinger C: The protein import machinery of mitochondria – a regulatory hub in metabolism, stress, and disease. Cell Metab, 2014; 19: 357-372.
  • Wenz LS, Opalinski L, Schuler MH, Ellenrieder L, Ieva R, Böttinger L, Qiu J, van der Laan M, Wiedemann N, Guiard B, PfannerN, Becker T: The presequence pathway is involved in protein sorting to the mitochondrial outer membrane. EMBO Rep, 2014; 15: 678-685.
  • Gold VAM, Ieva R, Walter A, Pfanner N, van der Laan M, Kühlbrandt W: Visualizing active membrane protein complexes by electron cryo-tomography. Nat. Commun, 2014; 5: 4129.
  • Pfanner N, van der Laan M, Amati P, Capaldi RA, Caudy AA, Chacinska A, Darshi M, Deckers M, Hoppins S, Icho T, Jakobs S, Ji J, Kozjak-Pavlovic V, Meisinger C, Odgren PR, Park SK, Rehling P, Reichert AS, Sheikh MS, Taylor SS, Tsuchida N, van der Bliek AM, van der Klei IJ, Weissman JS, Westermann B, Zha J, Neupert W, Nunnari J: Uniform nomenclature for the mitochondrial contact site and cristae organizing system. J. Cell Biol, 2014; 204: 1083-1086.



  • Gerbeth C, Schmidt O, Rao S, Harbauer AB, Mikropoulou D, Opalinska M, Guiard B, Pfanner N, Meisinger C: Glucose-induced regulation of protein import receptor Tom22 by cytosolic and mitochondria-bound kinases. Cell Metab, 2013; 18 (4) : 578-587: http://dx.doi.org/10.1016/j.cmet.2013.09.006
  • Ieva R, Heisswolf AK, Gebert M, Vogtle FN, Wollweber F, Mehnert CS, Oeljeklaus S, Warscheid B, Meisinger C, van der Laan M, Pfanner N: Mitochondrial inner membrane protease promotes assembly of presequence translocase by removing a carboxy-terminal targeting sequence. Nat Commun, 2013; 4: 2853-2853: http://dx.doi.org/10.1038/ncomms3853
  • Becker T, Horvath SE, Bottinger L, Gebert N, Daum G, Pfanner N: Role of phosphatidylethanolamine in the biogenesis of mitochondrial outer membrane proteins. J Biol Chem, 2013; 288 (23) : 16451-16459: http://dx.doi.org/10.1074/jbc.M112.442392
  • Qiu J, Wenz LS, Zerbes RM, Oeljeklaus S, Bohnert M, Stroud DA, Wirth C, Ellenrieder L, Thornton N, Kutik S, Wiese S, Schulze-Specking A, Zufall N, Chacinska A, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N, Wiedemann N, Becker T: Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. Cell, 2013; 154 (3) : 596-608: http://dx.doi.org/10.1016/j.cell.2013.06.033
  • van der Laan M, Schrempp SG, Pfanner N: Voltage-coupled conformational dynamics of mitochondrial protein-import channel. Nat Struct Mol Biol, 2013; 20 (8) : 915-917: http://dx.doi.org/10.1038/nsmb.2643
  • Wiedemann N, Stiller SB, Pfanner N: Activation and degradation of mitofusins: two pathways regulate mitochondrial fusion by reversible ubiquitylation. Mol Cell, 2013; 49 (3) : 423-425: http://dx.doi.org/10.1016/j.molcel.2013.01.027



Selected Previous Publications

  • Schmidt O, Harbauer AB, Rao S, Eyrich B, Zahedi RP, Stojanovski D, Schonfisch B, Guiard B, Sickmann A, Pfanner N, Meisinger C: Regulation of mitochondrial protein import by cytosolic kinases. Cell, 2011; 144 (2) : 227-239: http://dx.doi.org/10.1016/j.cell.2010.12.015
  • Stroud DA, Meisinger C, Pfanner N, Wiedemann N: Biochemistry. Assembling the outer membrane. Science, 2010; 328 (5980) : 831-832: http://dx.doi.org/10.1126/science.1190507
  • Chacinska A, Koehler CM, Milenkovic D, Lithgow T, Pfanner N: Importing mitochondrial proteins: machineries and mechanisms. Cell, 2009; 138 (4) : 628-644: http://dx.doi.org/10.1016/j.cell.2009.08.005
  • Wiedemann N, Meisinger C, Pfanner N: Cell biology. Connecting organelles. Science, 2009; 325 (5939) : 403-404: http://dx.doi.org/10.1126/science.1178016
  • Wiedemann N, van der Laan M, Pfanner N: SnapShot: import and sorting of mitochondrial proteins. Cell, 2009; 138 (4) : 808-808.e1: http://dx.doi.org/10.1016/j.cell.2009.08.004
  • Vögtle FN, Wortelkamp S, Zahedi RP, Becker D, Leidhold C, Gevaert K, Kellermann J, Voos W, Sickmann A, Pfanner N, Meisinger C: Global analysis of the mitochondrial N-proteome identifies a processing peptidase critical for protein stability. Cell, 2009; 139: 428-439.
  • Kutik, S., Stojanovski, D., Becker, L., Becker, T., Meinecke, M., Krüger, V., Prinz, C., Meisinger, C., Guiard, B., Wagner, R., Pfanner, N., and Wiedemann, N. (2008). Dissecting membrane insertion of mitochondrial β-barrel proteins. Cell 132, 1011-1024.
  • Meisinger, C., Sickmann, A., and Pfanner, N. (2008). The mitochondrial proteome: from inventory to function. Cell 134, 22-24.
  • Meinecke, M., Wagner, R., Kovermann, P., Guiard, B., Mick, D.U., Hutu, D.P., Voos, W., Truscott, K.N., Chacinska, A., Pfanner, N., and Rehling, P. (2006). Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science 312, 1523-1526.

  • Chacinska, A., Lind, M., Frazier, A.E., Dudek, J., Meisinger, C., Geissler, A., Sickmann, A., Meyer, H.E., Truscott, K.N., Guiard, B., Pfanner, N., and Rehling, P. (2005). Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120, 817-829.

  • Pfanner, N., Wiedemann, N., and Meisinger, C. (2004). Double membrane fusion. Science 305, 1723-1724.

  • Rehling, P., Model, K., Brandner, K., Kovermann, P., Sickmann, A., Meyer, H.E., Kühlbrandt, W., Wagner, R., Truscott, K.N., and Pfanner, N. (2003). Protein insertion into the mitochondrial inner membrane by a twin-pore translocase. Science 299, 1747-1751.

  • Wiedemann, N., Kozjak, V., Chacinska, A., Schönfisch, B., Rospert, S., Ryan, M.T., Pfanner, N., and Meisinger, C. (2003). Machinery for protein sorting and assembly in the mitochondrial outer membrane. Nature 424, 565-571.

  • Geissler, A., Chacinska, A., Truscott, K.N., Wiedemann, N., Brandner, K., Sickmann, A., Meyer, H.E., Meisinger, C., Pfanner, N., and Rehling, P. (2002). The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell 111, 507-518.

  • van Wilpe, S., Ryan, M.T., Hill, K., Maarse, A.C., Meisinger, C., Brix, J., Dekker, P.J.T., Moczko, M., Wagner, R., Meijer, M., Guiard, B., Hönlinger, A., and Pfanner, N. (1999). Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. Nature 401, 485-489.

  • Voisine, C., Craig, E.A., Zufall, N., von Ahsen, O., Pfanner, N., and Voos, W. (1999). The protein import motor of mitochondria: unfolding and trapping of preproteins are distinct and separable functions of matrix Hsp70. Cell 97, 565-574.

  • Hill, K., Model, K., Ryan, M.T., Dietmeier, K., Martin, F., Wagner, R., and Pfanner, N. (1998). Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature 395, 516-521.

  • Dietmeier, K., Hönlinger, A., Bömer, U., Dekker, P.J.T., Eckerskorn, C., Lottspeich, F., Kübrich, M., and Pfanner, N. (1997). Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature 388, 195-200.

  • Kiebler, M., Keil, P., Schneider, H., van der Klei, I.J., Pfanner, N., and Neupert, W. (1993). The mitochondrial receptor complex: a central role of MOM22 in mediating preprotein transfer from receptors to the general insertion pore. Cell 74, 483-492.

  • Pfanner, N., Rassow, J., van der Klei, I.J., and Neupert, W. (1992). A dynamic model of the mitochondrial protein import machinery. Cell 68, 999-1002.

  • Söllner, T., Rassow, J., Wiedmann, M., Schloßmann, J., Keil, P., Neupert, W., and Pfanner, N. (1992). Mapping of the protein import machinery in the mitochondrial outer membrane by crosslinking of translocation intermediates. Nature 355, 84-87.

  • Schneider, H., Söllner, T., Dietmeier, K., Eckerskorn, C., Lottspeich, F., Trülzsch, B., Neupert, W., and Pfanner, N. (1991). Targeting of the master receptor MOM19 to mitochondria. Science 254, 1659-1662.

  • Söllner, T., Pfaller, R., Griffiths, G., Pfanner, N., and Neupert, W. (1990). A mitochondrial import receptor for the ADP/ATP carrier. Cell 62, 107-115.

  • Kiebler, M., Pfaller, R., Söllner, T., Griffiths, G., Horstmann, H., Pfanner, N., and Neupert, W. (1990). Identification of a mitochondrial receptor complex required for recognition and membrane insertion of precursor proteins. Nature 348, 610-616.

  • Neupert, W., Hartl, F.-U., Craig, E.A., and Pfanner, N. (1990). How do polypeptides cross the mitochondrial membranes? Cell 63, 447-450.

  • Kang, P.-J., Ostermann, J., Shilling, J., Neupert, W., Craig, E.A., and Pfanner, N. (1990). Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins. Nature 348, 137-143.

  • Söllner, T., Griffiths, G., Pfaller, R., Pfanner, N., and Neupert, W. (1989). MOM19, an import receptor for mitochondrial precursor proteins. Cell 59, 1061-1070.

  • Pfanner, N., Orci, L., Glick, B.S., Amherdt, M., Arden, S.R., Malhotra, V., and Rothman, J.E. (1989). Fatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae. Cell 59, 95-102.

  • Pfanner, N., Tropschug, M., and Neupert, W. (1987). Mitochondrial protein import: nucleoside triphosphates are involved in conferring import-competence to precursors. Cell  49, 815-823.


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